Comparisonof NuclearNonhistonePhosphoproteinsof Rat Liver and NovikoftHepatoma1
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چکیده
The goal of the present study was to compare the nuclear phosphoproteins of normal and thioacetamide-treated rat liver, which are nondividing tissues, with regenerating rat liver and Novikoff hepatoma, which are growing and divid ing tissues. Thioacetamide treatment was utilized to induce nucleolar hypertrophy (1, 9). With the increased resolution of the 2-dimensional elec trophoresis method, (6, 49), the phosphoproteins soluble in 0.15 and 0.35 M NaCl were separated on polyacrylamide slab gels and subjected to autoradiography. In the Novikoff hepatoma patterns, there were 4 spots in the C-region, CU', C13p, C21p, and CMp, which were not found in the other tissues studied.
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